Disodium hydrogen phosphate dihydrate 99.5% or Disodium phosphate dihydrate or Sodium phosphate dibasic dihydrate or Sodium hydrogen orthophosphate dihydrate

Product Overview & Quality

Product Name: Disodium hydrogen phosphate dihydrate 99.5% or Disodium phosphate dihydrate or Sodium phosphate dibasic dihydrate or Sodium hydrogen orthophosphate dihydrate
Brand: QReC (Product Code: QR-D3243-1-0500)
Grade: Analytical Reagent High Purity Grade (AR Grade) featuring an exceptional certified chemical assay of not less than 99.5 percent. Manufactured and multi-crystallized under strict QReC global laboratory specifications, maintaining stoichiometric dihydrate hydration. Rigorously tested to enforce ultra-low ppm thresholds of chloride, sulfate, total nitrogen, water-insoluble matter, and trace heavy metals (such as iron, lead, copper, arsenic, zinc, calcium, and magnesium). Conforms to certified DNase, RNase, and Protease-free specifications, ensuring non-degrading integrity in sensitive molecular biology and biochemical workflows. Exhibits excellent crystal uniformity with low background ultraviolet absorbance, dissolving cleanly and rapidly in water without basic salt haziness. Packaged in a heavy-duty, moisture-barrier polymer container equipped with a secure hermetic seal.
Physical Properties: White crystalline powder or colorless to white crystalline granules. Odorless. Thermodynamically stable in ambient air with minimal caking tendencies compared to the anhydrous form. Aqueous solutions are mildly alkaline (pH approximately 9.0 to 9.3 for a 5 percent aqueous solution at room temperature). Non-combustible and non-corrosive solid.
Solubility: Freely and rapidly soluble in water (approximately 12 to 15 grams per 100 milliliters at room temperature; dissolves faster and more readily than the anhydrous counterpart, with solubility increasing substantially in warm water); practically insoluble in ethanol.
Key Use: Heavily utilized as the primary alkaline phosphate component paired with sodium dihydrogen phosphate to prepare physiological Phosphate Buffer (PB) and Phosphate-Buffered Saline (PBS) formulations (pH 5.8 to 8.0) for cell culture, molecular biology, immunology, and tissue culture maintenance. Extensively applied as a buffer modifier in High-Performance Liquid Chromatography (HPLC) mobile phases to stabilize analyte ionization and optimize chromatographic peak symmetry. Universally employed in protein purification protocols, enzyme kinetics, and nucleic acid extraction workflows. Serves as a standard analytical reagent and calibration reference for inorganic phosphate determinations in clinical diagnostics, water analysis, and pharmaceutical formulation testing.

Safety & Hazards

Classification: Non-hazardous substance under standard GHS criteria in typical laboratory usage. Exceptionally low acute toxicity profile; particulate dust may exert mild mechanical irritation to ocular, dermal, and respiratory surfaces.
Health Risks: Direct eye contact may cause transient mechanical stinging, conjunctival redness, or lacrimation. Prolonged dermal contact may produce mild dryness or superficial irritation. Inhalation of particulate dust can cause minor sneezing, coughing, or upper respiratory discomfort. Ingestion of excessive quantities may provoke transient gastrointestinal upset, abdominal cramps, or osmotic diarrhea due to phosphate salt accumulation.
First Aid Measures: Flush affected eyes thoroughly with clean running water for at least 10 to 15 minutes. Wash exposed skin with soap and water. In case of dust inhalation, relocate the individual to fresh air. If accidentally swallowed in substantial amounts, rinse mouth thoroughly, drink water, and seek medical advice if discomfort persists.

Handling & Storag

Storage: Stable Hydrated Buffer Salt. Must be preserved in its original, tightly closed QReC container. Store in a clean, strictly dry, cool, and well-ventilated dedicated chemical store at stable room temperature (below 25 to 30 degrees Celsius), sheltered from excessive humidity and direct solar radiation. Keep strictly segregated from strong mineral acids (which induce exothermic neutralization reactions) and strong oxidizing agents.
Precautions: Standard laboratory PPE is mandatory, including protective gloves, safety glasses, and a protective lab coat. Re-seal container cap tightly immediately after sampling. Minimize dust generation during weighing and dispensing.